期刊論文
| 學年 | 94 |
|---|---|
| 學期 | 1 |
| 出版（發表）日期 | 2005-08-01 |
| 作品名稱 | The Micro Patterning of Glutaraldehyde (GA)-Crosslinked Gelatin And Its Application To Cell-Culture |
| 作品名稱（其他語言） | |
| 著者 | Yang, Lung-jieh; Ou, Yu-cheng |
| 單位 | 淡江大學機械與機電工程學系 |
| 出版者 | Cambridge: RSC Publications |
| 著錄名稱、卷期、頁數 | Lab on a Chip 5(9), pp.979-984 |
| 摘要 | This paper proposes a novel technique for fabricating micro patterns of glutaraldehyde (GA)-crosslinked gelatin. It provides another means to crosslink gelatin other than using photo-sensitizing agents, and the micro patterns of GA-crosslinked gelatin can still be made successfully by accessing conventional photolithography. A much less toxic and increased biocompatible approach to strengthening the gelatin microstructures can be developed according to this idea. This paper also describes a potential methodology for using GA-crosslinked gelatin patterns as single-cell culture bases. The best spatial resolution of the micro gelatin bases can reach 10 µm, and the selective growing density of human Mesenchymal stem cells on the gelatin patterns surpasses the density on the glass substrate by 2–3 orders of magnitude. |
| 關鍵字 | collagen; gelatin; glutaraldehyde; photosensitizing agent; protein; biocompatibility; biotechnology; cell adhesion; cell culture; cell density; cell growth; chemical structure; conference paper; controlled study; cross linking; culture medium; human; human cell; hydrophilicity; mesenchyme; particle size; photolithography; priority journal; robotics; stem cell; surface property; technique; Biocompatible Materials; Cell Culture Techniques; Cross-Linking Reagents; Gelatin; Glass; Glutaral; Humans; Mesenchymal Stem Cells; Nanotechnology |
| 語言 | en |
| ISSN | 1473-0189 |
| 期刊性質 | 國際 |
| 收錄於 | SCI |
| 產學合作 | |
| 通訊作者 | Yang, Lung-Jieh |
| 審稿制度 | |
| 國別 | GBR |
| 公開徵稿 | |
| 出版型式 | 電子版 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/45667 ) |