Production, purification, and characterization of a cellulase from Paenibacillus elgii
學年 112
學期 2
出版（發表）日期 2024-07-17
作品名稱 Production, purification, and characterization of a cellulase from Paenibacillus elgii
作品名稱（其他語言）
著者 Wang, San-lang
單位
出版者
著錄名稱、卷期、頁數 Polymers 16(14),　2037
摘要 Cellulases are one of the most essential natural factors for cellulose degradation and, thus, have attracted significant interest for various applications. In this study, a cellulase from Paenibacillus elgii TKU051 was produced, purified, and characterized. The ideal fermentation conditions for cellulase productivity were 2% carboxymethyl cellulose (CMC) as the growth substrate, pH = 8, temperature of 31 °C, and 4 days of culturing. Accordingly, a 45 kDa cellulase (PeCel) was successfully purified in a single step using a High Q column with a recovery yield of 35% and purification of 42.2-fold. PeCel has an optimal activity at pH 6 and a temperature of 60 °C. The activity of cellulase was significantly inhibited by Cu2+ and enhanced by Mn2+. The PeCel-catalyzed products of the CMC hydrolysis were analyzed by high-performance liquid chromatography, which revealed chitobiose and chitotriose as the major products. Finally, the clarity of apple juice was enhanced when treated with PeCel.
關鍵字 cellulase;cellulose;Paenibacillus elgii
語言 en
ISSN 2073-4360
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 Wang, San-Lang
審稿制度 是
國別 CHE
公開徵稿
出版型式 ,電子版
相關連結

機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/125938 )

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