Bioconversion of peanut oil processing by-product into a novel α-glucosidase inhibitor: hemi-pyocyanin
學年 111
學期 2
出版（發表）日期 2023-05-12
作品名稱 Bioconversion of peanut oil processing by-product into a novel α-glucosidase inhibitor: hemi-pyocyanin
作品名稱（其他語言）
著者 Van Bon Nguyen, San-Lang Wang, Anh Dzung Nguyen
單位
出版者
著錄名稱、卷期、頁數 Processes 2023 11(5), 1468
摘要 Hemi-pyocyanin (HPC) is a heterocyclic nitrogenous compound with some reported potential medical effects. The current report aimed to investigate the potential use of organic industrial waste for the production of HPC via microbial fermentation. The novel antidiabetic activity of HPC was also accessed and reported in this work. A peanut oil processing by-product (groundnut cake) was screened as the best substrate for Pseudomonas aeruginosa TUN03 conversion to obtain high-yield HPC. This compound was further produced in a 14 L bioreactor system on a large scale (6 L per pilot) and reached higher productivity (35.1 μg/mL) in a shorter time course of cultivation (8 h) compared to fermentation on a small scale in flasks (19.5 μg/mL; 3 days of fermentation). On assessing its activity, HPC demonstrated potent inhibition against α-glucosidase, an antidiabetic enzyme, with a low IC50 value (0.572 mg/mL) and a maximum inhibition rate of 100%. In an in silico study, HPC was found to inhibit α-glucosidase with a good binding energy score (−9.0 kcal/mol) via interaction with amino acids Lys156, Leu313, and Arg315 at the active site, and three bonds (1 H-acceptor and 2 pi-H) were generated. The data from five Lipkin’s rules and ADMET-based pharmacokinetics and pharmacology revealed that HPC possesses drug-like properties and good ADMET properties within the required allotted limitations. The data obtained in the current work highlighted the potential application of groundnut cakes for the eco-friendly and scaled-up production of HPC, a new anti-α-glucosidase agent that should be further investigated for type 2 diabetes management.
關鍵字 diabetes;α-glucosidase inhibitors;hemi-pyocyanin;organic industrial waste;peanut oil processing by-product;fermentation;bioreactor systems
語言 en
ISSN 2227-9717
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 San-Lang Wang
審稿制度 是
國別 CHE
公開徵稿
出版型式 ,電子版
相關連結

機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/124109 )