Proteases production and chitin preparation from the liquid fermentation of chitinous fishery by-products by Paenibacillus elgii
學年 110
學期 1
出版（發表）日期 2021-08-25
作品名稱 Proteases production and chitin preparation from the liquid fermentation of chitinous fishery by-products by Paenibacillus elgii
作品名稱（其他語言）
著者 Lee DH; Doan CT; Tran TN; Nhuyen VB; Nguyen AD; Wang CL; Wang SL
單位
出版者
著錄名稱、卷期、頁數 Marine Drugs 19(9), 477
摘要 Chitinous fishery by-products have great application in the production of various bioactive compounds. In this study, Paenibacillus elgii TKU051, a protease-producing bacterial strain, was isolated using a medium containing 1% squid pens powder (SPP) as the sole carbon/nitrogen (C/N) source. P. elgii TKU051 was found to produce at least four proteases with molecular weights of 100 kDa, 57 kDa, 43 kDa, and 34 kDa (determined by the gelatin zymography method). A P. elgii TkU051 crude enzyme cocktail was optimally active at pH 6–7 and 60 °C. The 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and α-glucosidase inhibitory activity of the hydrolysates obtained from the hydrolysis of shrimp shell powder, shrimp head powder, shrimp meat powder, fish head powder and soya bean powder catalyzed by the P. elgii TkU051 crude enzyme cocktail were also evaluated. P. elgii TKU051 exhibited a high deproteinization capacity (over 94%) on different kinds of shrimp waste (shrimp heads and shells; fresh and cooked shrimp waste; shrimp waste dried by oven and lyophilizer), and the Fourier-transform infrared spectroscopy profile of the chitin obtained from the deproteinization process displayed the characteristic of chitin. Finally, the obtained chitin exhibited an effect comparable to commercial chitin in terms of adsorption against Congo Red (90.48% and 90.91%, respectively). Thus, P. elgii TKU051 showed potential in the reclamation of chitinous fishery by-products for proteases production and chitin extraction.
關鍵字 chitin;deproteinization;dye adsorption;Paenibacillus elgii;protease
語言 en
ISSN 1660-3397
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 WANG San-Lang
審稿制度 是
國別 CHE
公開徵稿
出版型式 ,電子版
相關連結

機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/121121 )

SDGS 消除貧窮,消除飢餓,潔淨水與衛生