Production of Sucrolytic Enzyme by Bacillus licheniformis by the Bioconversion of Pomelo Albedo as a Carbon Source
學年 109
學期 2
出版（發表）日期 2021-06-13
作品名稱 Production of Sucrolytic Enzyme by Bacillus licheniformis by the Bioconversion of Pomelo Albedo as a Carbon Source
作品名稱（其他語言）
著者 Chien Thang Doan; Thi Ngoc Tran; Thi Thanh Nguyen; Thi Phuong Hanh Tran; Van Bon Nguyen; Trung Dung Tran; Anh Dzung Nguyen; San-Lang Wang
單位
出版者
著錄名稱、卷期、頁數 Polymers 13(12), 1959
摘要 Recently, there has been increasing use of agro-byproducts in microbial fermentation to produce a variety of value-added products. In this study, among various kinds of agro-byproducts, pomelo albedo powder (PAP) was found to be the most effective carbon source for the production of sucrose hydrolyzing enzyme by Bacillus licheniformis TKU004. The optimal medium for sucrolytic enzyme production contained 2% PAP, 0.75% NH4NO3 , 0.05% MgSO4 , and 0.05% NaH2PO4 and the optimal culture conditions were pH 6.7, 35 ◦C, 150 rpm, and 24 h. Accordingly, the highest sucrolytic activity was 1.87 U/mL, 4.79-fold higher than that from standard conditions using sucrose as the carbon source. The purified sucrolytic enzyme (sleTKU004) is a 53 kDa monomeric protein and belongs to the glycoside hydrolase family 68. The optimum temperature and pH of sleTKU004 were 50 ◦C, and pH = 6, respectively. SleTKU004 could hydrolyze sucrose, raffinose, and stachyose by attacking the glycoside linkage between glucose and fructose molecules of the sucrose unit. The Km and Vmax of sleTKU004 were 1.16 M and 5.99 µmol/min, respectively. Finally, sleTKU004 showed strong sucrose tolerance and presented the highest hydrolytic activity at the sucrose concentration of 1.2 M–1.5 M.
關鍵字 agro-byproduct;Bacillus licheniformis;bioconversion;pomelo albedo;sucrolytic
語言 en
ISSN 2073-4360
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 WANG San-Lang
審稿制度 是
國別 CHE
公開徵稿
出版型式 ,電子版
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