A sensitive bithiophene-based biosensor for interferon-gamma characterization and analysis
學年 103
學期 1
出版（發表）日期 2014-11-28
作品名稱 A sensitive bithiophene-based biosensor for interferon-gamma characterization and analysis
作品名稱（其他語言）
著者 P.I. Tsai; S.S. Lee; S.T. Chou; C.M. Jan; Y.T. Chang; A.S.Y. Lee; C.K. Lee
單位
出版者
著錄名稱、卷期、頁數 Thin Solid Films 571(3), p.473-477
摘要 Real-time, label-free monitoring offers a wide range of applications to the biotech field. We developed an integrated bio-sensing platform which adapts a circular polarization interferometry configuration and a phase modulated ellipsometer to improve the performance of biomolecular measurements. An bithiophene-based conductive linker-5′-(mercapto)-[2, 2′-bithiophene]-5-carboxylic acid was developed for detecting antibody–antigen interactions. In this study, the biomarker interferon-gamma (IFN-γ), one of the most important indicators of tuberculosis, was chosen to test and verify the sensitivity of our measurement system. Our experimental results showed that an increase in the concentration of the IFN-γ (64 pM to 1 μM) was usually accompanied by a phase increase. This result is good evidence that our biosensing system can be useful for analyzing biomolecular interactions.
關鍵字 Surface plasmon resonance;Ellipsometry;Interferon-gamma;Phase-interrogation;Bithiophene
語言 en
ISSN 1879-2731
期刊性質 國外
收錄於 SCI
產學合作
通訊作者
審稿制度 否
國別 NLD
公開徵稿
出版型式 ,電子版
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