Deletion of the Carboxyl-Terminal Residue Disrupts the Amino-Terminal Folding, Self-Association and Thermal Stability of an Amphipathic Antimicrobial Peptide
學年 107
學期 2
發表日期 2019-05-29
作品名稱 Deletion of the Carboxyl-Terminal Residue Disrupts the Amino-Terminal Folding, Self-Association and Thermal Stability of an Amphipathic Antimicrobial Peptide
作品名稱（其他語言）
著者 Lee, Chang-shin
作品所屬單位
出版者
會議名稱 The 24th Biophysics Conference
會議地點 宜蘭縣，台灣
摘要 Understanding the complex relationship between amino acid sequence and protein behaviors, such as folding and self-association, is a major goal of protein research. In the present work, we examined the effects of deleting a C-terminal residue on the intrinsic properties of an amphapathic α-helix of mastoparan-B (MP-B), an antimicrobial peptide with the sequence LKLKSIVSWAKKVL-NH2. We used circular dichroism and nuclear magnetic resonance to demonstrate that the peptide MP-B([1-13]) displayed significant unwinding at the N-terminal helix compared with the parent peptide of MP-B, as the temperature increased when the residue at position 14 was deleted. Pulsed-field gradient nuclear magnetic resonance data revealed that MP-B forms a larger diffusion unit than MP-B([1-13]) at all experimental temperatures and continuously dissociates as the temperature increases. In contrast, the size of the diffusion unit of MP-B([1-13]) is almost independent of temperature. These findings suggest that deleting the flexible, hydrophobic amino acid from the C-terminus of MP-B is sufficient to change the intrinsic helical thermal stability and self-association. This effect is most likely because of the modulation of enthalpic interactions and conformational freedom that are specified by this residue. Our results implicate terminal residues in the biological function of an antimicrobial peptide.
關鍵字 antimicrobial peptide;diffusion;enthalpic interaction;self-association;thermal stability
語言 zh_TW
收錄於
會議性質 國內
校內研討會地點 無
研討會時間 20190529~20190601
通訊作者
國別 TWN
公開徵稿
出版型式
出處
相關連結

機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/120403 )

SDGS 優質教育