Production of potent antidiabetic compounds from shrimp head powder via Paenibacillus conversion
學年 107
學期 1
出版（發表）日期 2019-01-01
作品名稱 Production of potent antidiabetic compounds from shrimp head powder via Paenibacillus conversion
作品名稱（其他語言）
著者 Van Bon Nguyen; San-Lang Wang
單位
出版者
著錄名稱、卷期、頁數 Process Biochemistry 76, p.18-24
摘要 Natural α-glucosidase inhibitors (aGIs) are of great interest as an efficacious and safe therapy for type 2 diabetes, which is an ongoing global health issue. The aim of this study is to utilize shrimp head powder (SHP), an abundant and low-cost material, for the biosynthesis, isolation, and identification of active antidiabetic compounds. SHP was efficiently converted to aGIs via Paenibacillus sp. TKU042 fermentation. Fermented SHP (fSHP) by this strain possesses high pH stability, and stronger yeast α-glucosidase inhibitory activity (92%) than that of acarbose (60%). aGI productivity increased more than 2-fold after optimization (from 225 U/mL to 560 U/mL). Further bioactivity-guided isolation of two major active compounds were identified as nicotinic acid and adenine. Notably, these inhibitors were non-sugar-based moiety aGIs, which is newly isolated and identified from fSHP in this study. In the tests of specific enzyme inhibitory activity, adenine showed highly specific inhibition against yeast α-glucosidase (IC50 = 22 μg/mL); nicotinic acid demonstrated good effect on rat α-glucosidase (IC50 = 70 μg/mL); while acarbose possessed efficient effect on bacteria, rice, and rat α-glucosidases (IC50 = 0.03–108 μg/mL). The current results suggest that it is cost-effective to produce potent aGIs from SHP via Paenibacillus conversion, and these active constituents may be useful in type 2 diabetes management.
關鍵字 Shrimp heads;Marine processing;Antidiabetes;Paenibacillus;Nicotinic acid, Adenine
語言 en
ISSN 1359-5113
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 San-Lang Wang
審稿制度 是
國別 USA
公開徵稿
出版型式 ,電子版
相關連結

機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/115716 )