Enhanced Signal and Quantitative Detection of nti-Interferon-Gamma Antibody by Using a Nanometer Biolinker
學年 104
學期 2
出版（發表）日期 2016-07-26
作品名稱 Enhanced Signal and Quantitative Detection of nti-Interferon-Gamma Antibody by Using a Nanometer Biolinker
作品名稱（其他語言）
著者 Pei-I Tsai; Adam Shih-Yuan Lee; Shu-Sheng Lee; Ming-Han Chung; Meng-Wei Liu; Chih-Kung Lee
單位
出版者
著錄名稱、卷期、頁數 PLoS ONE 11(7)
摘要 For rapid screening and quantification of an antisera antibody, a nanometer bithiophene-based conductive biolinker can enhanced signal performance and can be used to verify the interaction of an anti-IFN-γ antibody with an IFN-γ protein. The experimental measurements take a generic approach which takes advantage of the functionality of thiophene-based linkers for biosensors. Effects associated with using bithiophene as a biolinker for surface plasmon resonance (SPR) spectroscopy are examined in this paper. By using an atomic force microscope (AFM), it was observed that the morphology of the bithiophene modified gold sensor surface became smoother than the original gold surface. We compared the response and concentration of the anti-IFN-γ antibody on a bithiophene-coated and dextran-coated biochip as well as on different thickness-modified surfaces under SPR relevant conditions. The results indicate that a response to IFN-γ molecules immobilized on a sensor using a bithiophene biolinker improved more than 8-fold when compared to that of a sensor using a dextran biolinker. Furthermore, the regeneration ability of the sensor surface shows good repeatability as only less than a 1% decrease was found after repeating the experimental work over 6 cycles. The characteristics provided us with a good platform for rapid screening, real-time monitoring and quantitative concentration of the autoimmune antibody activities.
關鍵字
語言 en_US
ISSN
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 Chih-Kung Lee
審稿制度 是
國別 USA
公開徵稿
出版型式 ,電子版
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機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/111872 )

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