Application of chitinous materials in production and purification of a poly(L-lactic acid) depolymerase from Pseudomonas tamsuii TKU015 | |
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學年 | 104 |
學期 | 2 |
出版(發表)日期 | 2016-03-22 |
作品名稱 | Application of chitinous materials in production and purification of a poly(L-lactic acid) depolymerase from Pseudomonas tamsuii TKU015 |
作品名稱(其他語言) | |
著者 | Tzu-Wen Liang; Shan-Ni Jen; Anh Dzung Nguyen; San-Lang Wang |
單位 | |
出版者 | |
著錄名稱、卷期、頁數 | Polymers 8(3), pp.98(11 pages) |
摘要 | The management of fishery residues and plastics is considered to be a vital strategy for conserving resources and maintaining the quality of the environment. Poly(l-lactic acid) (PLA) is a commercially promising, renewable, and biodegradable plastic. In this study, a PLA depolymerase was produced in a squid pen powder (SPP) and recycled plastic waste (PLA powder)-containing medium by Pseudomonas tamsuii TKU015, a bacterial strain isolated from Taiwanese soil. This PLA depolymerase had a molecular weight of 58 kDa and was purified to homogeneity from the supernatant of a TKU015 culture. The optimum pH of TKU015 PLA depolymerase is 10, and the optimal temperature of the enzyme is 60 °C. In addition to PLA, TKU015 PLA depolymerase degraded fibrinogen and tributyrin, but did not hydrolyze casein, triolein, and poly(β-hydroxybutyrate). Taken together, these data demonstrate that P. tamsuii TKU015 produces a PLA depolymerase to utilize SPP and polylactide as carbon/nitrogen sources. |
關鍵字 | PLA;squid pen;Pseudomonas tamsuii;PLA depolymerase;degradation |
語言 | en |
ISSN | 2073-4360 |
期刊性質 | 國外 |
收錄於 | SCI |
產學合作 | |
通訊作者 | San-Lang Wang |
審稿制度 | 是 |
國別 | CHE |
公開徵稿 | |
出版型式 | ,電子版 |
相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/107110 ) |