Evaluation of the teratogenic effects of three traditional Chinese medicines, Si Jun Zi Tang, Liu Jun Zi Tang and Shenling Baizhu San, during zebrafish pronephros development
學年 104
學期 1
出版（發表）日期 2015-12-11
作品名稱 Evaluation of the teratogenic effects of three traditional Chinese medicines, Si Jun Zi Tang, Liu Jun Zi Tang and Shenling Baizhu San, during zebrafish pronephros development
作品名稱（其他語言）
著者 Ding, Yu-Ju; Wang, Bo-Cheng; Wen, Chi-Chung; Sun, Chiao-Yin; Lee, Hsun-Hua; Lee, Fei-Peng; Yang, Ling-Ling; Chen, Yau-Hung
單位
出版者
著錄名稱、卷期、頁數 Journal of Toxicologic Pathology 28(3), p.141-149
摘要 The aim of this study was to evaluate the teratogenic effects of three common Chinese medical prescriptions, Si Jun Zi Tang (SJZT), Liu Jun Zi Tang (LJZT) and Shenling Baizhu San (SLBS), during zebrafish pronephros development. We used the transgenic zebrafish line Tg(wt1b:EGFP) to assess the teratogenic effects using 12 different protocols, which comprised combinations of 4 doses (0, 25, 250, 1,250 ng/mL) and 3 exposure methods [methods I, 12–36 hours post fertilization (hpf), II, 24–48 hpf, and III, 24–36 hpf]. As a result, few defects in the kidneys were observed in the embryos exposed to 25 ng/mL of each medical prescription. The percentage of kidney malformation phenotypes increased as the exposure concentrations increased (25 ng/mL, 0–10%; 250 ng/mL, 0–60%; 1,250 ng/mL, 80–100%). Immunohistochemistry for α6F, which is a basolateral and renal tubular differentiation marker, revealed no obvious defective phenotypes in either SJZT- or LJZT-treated embryos, indicating that these Chinese medical prescriptions had minimal adverse effects on the pronephric duct. However, SLBS-treated embryos displayed a defective phenotype in the pronephric duct. According to these findings, we suggest (1) that the Chinese medical prescriptions induced kidney malformation phenotypes that are dose dependent and (2) that the embryonic zebrafish kidney was more sensitive to SLBS than SJZT and LJZT.
關鍵字 Chinese medical prescriptions;kidney;nephrotoxicity;zebrafish
語言 en
ISSN 1881-915X ; 0914-9198
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 Yang, Ling-Ling; Chen, Yau-Hung
審稿制度 是
國別 JPN
公開徵稿
出版型式 ,電子版,紙本
相關連結

機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/105932 )