Purification of chitinase/chitosanase from Bacillus cereus and discovery of an enzyme inhibitor
學年 102
學期 1
出版（發表）日期 2013-10-26
作品名稱 Purification of chitinase/chitosanase from Bacillus cereus and discovery of an enzyme inhibitor
作品名稱（其他語言）
著者 Liang, Tzu-Wen; Chen, Yue-Yin; Pan, Po-Shen; Wang, San-Lang
單位 淡江大學化學學系
出版者 Amsterdam: Elsevier BV
著錄名稱、卷期、頁數 International Journal of Biological Macromolecules 63, pp.8-14
摘要 A chitinase and a chitosanase were induced from a squid pen powder (SPP)-containing medium of Bacillus cereus TKU030 and purified by precipitation with ammonium sulphate and combined column chromatography. The purified chitinase and chitosanase exhibited optimum activity at 60 °C, pH 5–6 and 40 °C, pH 4, respectively. The chitinase and chitosanase were stable at 25–60 °C, pH 4–7 and 25–50 °C, pH 3–7, respectively. The chitinase and chitosanase showed the highest activity toward β-chitin and 60% DD chitosan, respectively. The chitinase was significantly inhibited by Mn2+ and EDTA but activated by Cu2+, Fe2+ and Ca2+. The chitosanase was significantly inhibited by Cu2+, Fe2+, Zn2+, Mn2+ and EDTA. The chitinase showed high stability in the presence of various surfactants, such as SDS, Tween 20, Tween 40 and Triton X-100. In contrast, these surfactants were inhibitors of the chitosanase. The chitinase and chitosanase were also inhibited by TKUPSP017, a small synthetic boron-containing molecule with a BF3K side-chain. However, TKUPSP017 enhanced the growth of B. cereus TKU030 in SPP-containing medium.
關鍵字 Bacillus cereus;Boron;Chitinase;Chitinase inhibitor;Chitosanase;Squid pen
語言 en
ISSN 1879-0003
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 Wang, San-Lang
審稿制度 是
國別 NLD
公開徵稿
出版型式 ,電子版,紙本
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