Production and purification of a fungal chitosanase and chitooligomers from Penicillium janthinellum D4 and discovery of the enzyme activators
學年 103
學期 1
出版（發表）日期 2014-08-08
作品名稱 Production and purification of a fungal chitosanase and chitooligomers from Penicillium janthinellum D4 and discovery of the enzyme activators
作品名稱（其他語言）
著者 Anh Dzung Nguyen; Huang, Chun-Ching; Liang, Tzu-Wen; Van Bon Nguyen; Pan, Po-Shen; Wang, San-Lang
單位 淡江大學化學學系
出版者 Kidlington: Pergamon Press
著錄名稱、卷期、頁數 Carbohydrate Polymers 108, pp.331–337
摘要 Chitosanases have received much attention because of their wide range of applications. Although most fungal chitosanases use sugar as their major carbon source, in the present work, a chitosanase was induced from a squid pen powder (SPP)-containing Penicillium janthinellum D4 medium and purified by ammonium sulphate precipitation and combined column chromatography. The purified D4 chitosanase exhibited optimum activity at pH 7–9, 60 °C and was stable at pH 7–11, 25–50 °C. The D4 chitosanase that was used for chitooligomers preparation was studied. The enzyme products revealed various chitooligomers with different degrees of polymerisation (DP) from 3 to 9, as determined by a MALDI-TOF mass spectrometer, confirming the endo-type nature of the D4 chitosanase. D4 chitosanase activity was significantly inhibited by Cu2+, Mn2+, and EDTA. However, Fe2+ activated or inhibited D4 chitosanases at different concentrations. The D4 chitosanase was also activated by some small synthetic boron-containing molecules with boronate ester side chains.
關鍵字 Chitosanase; Squid pen; Penicillium janthinellum; Chitooligomers; Chitosanase activator; Boron
語言 en
ISSN 0144-8617
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 Wang, San-Lang
審稿制度 是
國別 GBR
公開徵稿
出版型式 ,紙本
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