A novel nattokinase produced by Pseudomonas sp. TKU015 using shrimp shells as substrate
學年 97
學期 1
出版（發表）日期 2009-01-01
作品名稱 A novel nattokinase produced by Pseudomonas sp. TKU015 using shrimp shells as substrate
作品名稱（其他語言）
著者 Wang, San-Lang; Chen, Hsin-Jen; Liang, Tzu-Wen; Lin, Yung-Di
單位 淡江大學生命科學研究所
出版者 Camden: Elsevier Ltd
著錄名稱、卷期、頁數 Process Biochemistry 44(1), pp.70-76
摘要 A nattokinase was purified from the culture supernatant of Pseudomonas sp. TKU015 with shrimp shell wastes as the sole carbon/nitrogen source. The molecular masses of TKU015 nattokinase determined by SDS-PAGE and gel filtration were approximately 21 and 24 kDa, respectively. The optimum pH, optimum temperature, pH stability, and thermal stability of TKU015 nattokinase were 7, 50 °C, pH 4–11, and less than 50 °C, respectively. TKU015 nattokinase was inhibited completely by PMSF, indicating that the TKU015 nattokinase was serine protease. The results of peptide mass mapping showed that two tryptic peptides of the nattokinase were identical to a chitin binding protein from Bacillus cereus ATCC 14579 (GenBank accession number gi30020946) with 23% sequence coverage. With this method, Pseudomonas sp. TKU015 produces a nattokinase/fibrinolytic enzyme and may be considered as a new source for thrombolytic agents.
關鍵字 Fibrinolytic enzyme; Nattokinase; Pseudomonas sp.; Shrimp shell wastes
語言 en
ISSN 1359-5113
期刊性質 國外
收錄於 SCI EI
產學合作
通訊作者 Wang, San-Lang
審稿制度
國別 USA
公開徵稿
出版型式 紙本
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