Bioconversion of shellfish chitin wastes for the production of Bacillus subtilis W-118 chitinase
學年 95
學期 1
出版（發表）日期 2006-11-01
作品名稱 Bioconversion of shellfish chitin wastes for the production of Bacillus subtilis W-118 chitinase
作品名稱（其他語言）
著者 Wang, San-Lang; Lin, Tzu-Yin; Yen, Yue-Horng; Liao, Hui-Fen; Chen, Yu-Jen
單位 淡江大學生命科學研究所
出版者 Kidlington: Pergamon
著錄名稱、卷期、頁數 Carbohydrate Research 341(15), pp.2507-2515
摘要
關鍵字 Antifungal; Antitumor; Chitin oligosaccharides; Chitinase; Bacteria; Bioconversion; Disease control; Enzymes; pH effects; Polysaccharides; Tumors; Wastes; Chitin; chitin; chitinase; unclassified drug; w 118 chitinase; analytic method; article; Bacillus subtilis; biotransformation; chromatography; controlled study; crab; fungal strain; Fusarium oxysporum; high performance liquid chromatography; incubation time; leukemia cell; molecular weight; nonhuman; pH; polymerization; priority journal; shellfish; shrimp; temperature; Animals; Antifungal Agents; Bacillus subtilis; Bacterial Proteins; Cell Division; Chitinase; Crustacea; Humans; Hydrogen-Ion Concentration; K562 Cells; Kinetics; Waste Products; Bacillus subtilis; Decapoda (Crustacea); Fusarium oxysporum
語言 en
ISSN 0008-6215
期刊性質 國外
收錄於 SCI EI
產學合作
通訊作者
審稿制度
國別 GBR
公開徵稿
出版型式 Bacillus subtilis W-118, a strain that produces antifungal materials, excreted a chitinase when cultured in a medium containing shrimp- and crab-shell powder as the major carbon source. This chitinase, purified by sequential chromatography, had a molecular mass of 20,600 Da and a pI of 6. The optimum pH, optimum temperature, and pH stability of the chitinase were pH 6, 37 °C, and pH 5–7, respectively. The unique characteristics of the purified chitinase include low molecular mass and acidic pI. In the investigation of the inhibitory activity, it was found that the growth of Fusarium oxysporum was 100% inhibited after incubation for 1 day with sterilized W-118 chitinase solution (5.6 units/mL). The chitinase hydrolyzates of chitin with low degrees of polymerization (DP 1–6) were analyzed by HPLC. Longer reaction times led to the generation of chitin oligosaccharides with lower DP. The chitin oligosaccharides were examined for their inhibitory effects on F. oxysporum and human leukemia cell lines. 紙本
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