Purification and characterization of a novel catechol 1,2-dioxygenase from Pseudomonas aeruginosa with benzoic acid as a carbon source
學年 94
學期 2
出版（發表）日期 2006-07-01
作品名稱 Purification and characterization of a novel catechol 1,2-dioxygenase from Pseudomonas aeruginosa with benzoic acid as a carbon source
作品名稱（其他語言）
著者 Wang, Chuan-Lu; You, Su-Lin; Wang, San-Lang
單位 淡江大學生命科學研究所
出版者 Camden: Elsevier Ltd
著錄名稱、卷期、頁數 Process Biochemistry 41(7), pp.1594-1601
摘要 Pseudomonas aeruginosa TKU002, capable of growing significantly on acidic side pH 5.5 and benzoic acid as a sole carbon source, was isolated from enrichment culture accumulation of catechol. An intracellular catechol 1,2-dioxygenase (CD) produced by P. aeruginosa TKU002 was purified and characterized. The TKU002 CD was found to have unique characteristics different to other microbial CDs. The unique characteristics include the smallest molecular mass of 22 kDa, the most acidic pI value of lower than 4, and the highest cleavage activity to the substrate, pyrogallol. Different to other CD producing strains, P. aeruginosa TKU002 produced CD at a lower pH of 5.5 when benzoate was used as the sole carbon source. The TKU002 CD was a monomer with a Km of 5.9 μM. The TKU002 CD showed 36% and 14% sequence coverage rate with protocatechuate 3,4-dioxygenase β-subunit of P. aeruginosa UCBPP-COG3485 and catechol 1,2-dioxygenase of P. aeruginosa PA01, respectively, and possessed one matched peptide (YLWDDFAYATR). In conclusion, this is the first report of a microbial CD with the smallest molecular mass, the most acidic pI value, the highest specific activity to the substrate pyrogallol, and the most acidic medium for CD production.
關鍵字 Amino acids; Aromatic hydrocarbons; Bacteria; Bioconversion; Characterization; Enzymes; Monomers; pH; Benzoate-assimilating bacterium; Catechol 1,2-dioxygenase; Catechol production; Pyrogallol; Enzyme kinetics; Bacteria (microorganisms); Pseudomonas aeruginosa
語言 en
ISSN 1359-5113
期刊性質 國外
收錄於 SCI EI
產學合作
通訊作者
審稿制度
國別 USA
公開徵稿
出版型式
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