| Conversion of squid pen by using Serratia sp. TKU020 fermentation for the production of enzymes, antioxidants, and N-acetyl chitooligosaccharides | |
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| 學年 | 98 |
| 學期 | 1 |
| 出版（發表）日期 | 2009-08-01 |
| 作品名稱 | Conversion of squid pen by using Serratia sp. TKU020 fermentation for the production of enzymes, antioxidants, and N-acetyl chitooligosaccharides |
| 作品名稱（其他語言） | |
| 著者 | Wang, San-Lang; Liou, Jen-Yi; Liang, Tzu-Wen; Liu, Kao-Cheng |
| 單位 | 淡江大學化學學系 |
| 出版者 | Camden: Elsevier Ltd |
| 著錄名稱、卷期、頁數 | Process Biochemistry 44(8), pp.854-861 |
| 摘要 | A chitinase (CHT), a chitosanase (CHS) and a protease (PRO) were purified from the culture supernatant of Serratia sp. TKU020 with squid pen as the sole carbon/nitrogen source. The molecular masses of CHT, CHS and PRO determined by SDS-PAGE were approximately 65 kDa, 55 kDa and 55 kDa, respectively. CHT and CHS were inhibited by Mn2+, EDTA and PRO was inhibited by Mg2+, EDTA. The antioxidant activity of TKU020 culture supernatant was 78% (DPPH scavenging ability). N-Acetylglucosamine (GlcNAc) and N-acetyl chitobiose (GlcNAc)2 were also produced from the culture supernatant by using TKU020 strain fermentation. The maximum production of GlcNAc and (GlcNAc)2 was 1.3 mg/mL and 2.7 mg/mL, respectively, after 4 days of fermentation. With this method, we have shown that squid pen wastes can be utilized and it is effective in the production of enzymes, antioxidants, and N-acetyl chitooligosaccharides, facilitating its potential use in industrial applications and functional foods. |
| 關鍵字 | Chitinase; Chitosanase; Protease; Antioxidant activity; N-Acetyl chitooligosaccharides; Serratia sp. |
| 語言 | en |
| ISSN | 1359-5113 |
| 期刊性質 | 國外 |
| 收錄於 | SCI |
| 產學合作 | |
| 通訊作者 | |
| 審稿制度 | |
| 國別 | USA |
| 公開徵稿 | |
| 出版型式 | |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/18873 ) |
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