| Degradation of chitin and production of bioactive materials by bioconversion of squid pens | |
|---|---|
| 學年 | 98 |
| 學期 | 1 |
| 出版（發表）日期 | 2009-09-01 |
| 作品名稱 | Degradation of chitin and production of bioactive materials by bioconversion of squid pens |
| 作品名稱（其他語言） | |
| 著者 | Wang, San-Lang; Yang, Chiann-Wen; Liang, Tzu-Wen; Peng, Juo-Hua; Wang, Chuan-Lu |
| 單位 | 淡江大學化學學系 |
| 出版者 | Kidlington: Pergamon |
| 著錄名稱、卷期、頁數 | Carbohydrate Polymers 78(2), pp.205-212 |
| 摘要 | Serratia marcescens TKU011, a protease- and chitosanase-producing bacterium, the optimized condition for protease and chitosanase production was found after the media were heated at 121 °C for 120 min and the culture was shaken at 25 °C for 5 days in 100 mL of medium containing 1% squid pen powder (SPP) (w/v), 0.1% K2HPO4, and 0.05% MgSO4. An extracellular metalloprotease with novel properties of solvent stable, and alkaline was purified from the culture supernatant of S. marcescens TKU011 with squid pen wastes as the sole carbon/nitrogen source. The enzyme was a monomeric protease with a molecular mass of 48–50 kDa by SDS–PAGE and gel filtration chromatography. The optimum pH, optimum temperature, pH stability, and thermal stability of TKU011 protease were 8, 50 °C, pH 5–11, and <40 °C, respectively. Besides protease and chitosanase, with this method, deproteinization of squid pen for β-chitin, the production of peptide and reducing sugar may be useful for biological applications. |
| 關鍵字 | Serratia marcescens; Protease; Chitosanase; Squid pen wastes; Deproteinization |
| 語言 | en |
| ISSN | 0144-8617 |
| 期刊性質 | 國外 |
| 收錄於 | SCI |
| 產學合作 | |
| 通訊作者 | |
| 審稿制度 | |
| 國別 | GBR |
| 公開徵稿 | |
| 出版型式 | |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/18850 ) |
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