Biodegradation of shellfish wastes and production of chitosanases by a squid pen-assimilating bacterium, Acinetobacter calcoaceticus TKU024
學年 100
學期 1
出版（發表）日期 2011-09-01
作品名稱 Biodegradation of shellfish wastes and production of chitosanases by a squid pen-assimilating bacterium, Acinetobacter calcoaceticus TKU024
作品名稱（其他語言）
著者 Wang, San-Lang; Tseng, Wan-Nine; Liang, Tzu-Wen
單位 淡江大學生命科學研究所; 淡江大學化學學系
出版者 Dordrecht: Springer Netherlands
著錄名稱、卷期、頁數 Biodegradation 22(5), pp.939-948
摘要 Two chitosanases (CHSA1 and CHSA2) were purified from the culture supernatant of Acinetobacter calcoaceticus TKU024 with squid pen as the sole carbon/nitrogen source. The molecular masses of CHSA1 and CHSA2 determined by SDS-PAGE were approximately 27 and 66 kDa, respectively. The optimum pH, optimum temperature, pH stability, and thermal stability of CHSA1 and CHSA2 were (pH 6, 50°C, pH 4–10, ＜90°C) and (pH 7, 60°C, pH 6–11, ＜70°C), respectively. CHSA1 and CHSA2 had broad pH and thermal stability. CHSA1 and CHSA2 were both inhibited by EDTA and were inhibited completely by 5 mM Mn2+. CHSA1 and CHSA2 degraded chitosan with DD ranging from 60 to 98%, and also degraded some chitin. The most susceptible substrate was 60% deacetylated chitosan. Furthermore, TKU024 culture supernatant (1.5% SPP) incubated for 5 days has the most reducing sugars (0.63 mg/ml). With this method, we have shown that shellfish wastes may have a great potential for the production of bioactive materials.
關鍵字 Chitosanase;Squid pen wastes;Acinetobacter calcoaceticus;Reducing sugar
語言 en_US
ISSN 1572-9729 0923-9820
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 Wang, San-Lang
審稿制度 是
國別
公開徵稿
出版型式 電子版 紙本
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