Applied development of crude enzyme from Bacillus cereus in prebiotics and microbial community changes in soil
學年 101
學期 2
出版(發表)日期 2013-02-15
作品名稱 Applied development of crude enzyme from Bacillus cereus in prebiotics and microbial community changes in soil
作品名稱(其他語言)
著者 Liang, Tzu-Wen; Liu, Chin-Pei; Wu, Chunhung; Wang, San-Lang
單位 淡江大學化學學系
出版者 Kidlington: Pergamon
著錄名稱、卷期、頁數 Carbohydrate Polymers 92(2), pp.2141–2148
摘要 The chitosanase and chitinase activity were revealed in the culture supernatant of Bacillus cereus TKU027 with shrimp head powder (SHP) as the sole carbon/nitrogen source. The chitosan with 60% degree of deacetylation (DD) was depolymerized by TKU027 crude enzyme. The low DP oligomers stimulated the growth of Lactobacillus paracasei BCRC12193 and Lactobacillus kefir BCRC14011 in a MRS broth supplemented with low DP oligomers for 12 h. Conversely, the high DP oligomers (0.1%) had potent inhibitory effects against L. paracasei BCRC12193 and L. kefir BCRC14011 for 48 h. Besides, the study also investigated the effects of B. cereus TKU027 on degradation of SHP and the survival conditions of bacteria in mangrove river sediment of Tamsui River. The 5 weeks-incubation sample of SHP and B. cereus TKU027-amended mangrove river sediment showed the highest amounts of biomass, reducing sugar and total sugar, and some variance of bacterial community compositions existed in the soils.
關鍵字 Chitosanase; Chitosan oligosaccharides; Depolymerization; Bacillus cereus; Prebiotic; PCR-DGGE
語言 en
ISSN 0144-8617
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 Wang, San-Lang
審稿制度
國別 GBR
公開徵稿
出版型式 電子版
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