Reclamation of squid pen by Bacillus licheniformis TKU004 for the production of thermally stable and antimicrobial biosurfactant
學年 100
學期 1
出版（發表）日期 2012-01-01
作品名稱 Reclamation of squid pen by Bacillus licheniformis TKU004 for the production of thermally stable and antimicrobial biosurfactant
作品名稱（其他語言）
著者 Chen, Yu-chi; Chiang, Tsung-ju; Liang, Tzu-wen; Wang I-li; Wang, San-lang
單位 淡江大學化學學系; 淡江大學生命科學開發中心
出版者 Elsevier
著錄名稱、卷期、頁數 Biocatalysis and Agricultural Biotechnology 1(1), pp.62-69
摘要 Bacillus licheniformis TKU004 produced biosurfactants in a medium with 1% squid pen powder as the sole carbon/nitrogen source at 37 °C for 1 day by using a baffled base flask, showing a growth-associated production. The biosurfactant was extracted from the culture supernatant by acid precipitation, extraction of ethyl acetate and methanol with the yield of 0.55 g/L. The biosurfactant could reduce surface tension of water from 72.30 to 25.42 mN/m at a concentration of 350 mg/L and the emulsification index of machine oil reached 68% after 24 h. The biosurfactant showed stability including thermal (121 °C, 20 min) and pH (7–11) stability regarding the surface tension reduction and the emulsification capacity of machine oil, and tolerance under salt concentrations (<2%). The biosurfactant also showed antimicrobial activity significantly against cell growth of E. coli BCRC13086 and S. aureus BCRC10780. The results obtained in this work are noteworthy for possible biosurfactant production from SPP with potential of application in removal of oil and in the future to gasoline-contaminated ocean shores for ecological protection purposes. Besides, it is also possible to be applied as a biocontrol agent.
關鍵字 Bacillus licheniformis;Biosurfactant;Surface tension;Emulsification;Squid pen
語言 en
ISSN 1878-8181
期刊性質 國外
收錄於 SCI
產學合作
通訊作者
審稿制度 是
國別 NLD
公開徵稿
出版型式 ,電子版
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