A novel structural specific creatinine sensing scheme for the determination of the urine creatinine
學年 100
學期 1
出版（發表）日期 2012-01-01
作品名稱 A novel structural specific creatinine sensing scheme for the determination of the urine creatinine
作品名稱（其他語言）
著者 Chen, Chi-Hao; Lin, Meng-Shan
單位 淡江大學化學學系
出版者 Amsterdam: Elsevier BV
著錄名稱、卷期、頁數 Biosensors and Bioelectronics 31(1), pp.90–94
摘要 In this work, a highly structural dependent amperometric scheme was proposed for the determination of creatinine without enzymatic assistance. The principle of this novel method is based upon the formation of a soluble copper–creatinine complex on the copper electrode surface. Subsequently, an oxidative current from the regeneration of the surface oxide layer is monitored and it is proportional to the concentration of the creatinine. This scheme can be conducted at potential of −0.1 V (vs. Ag/AgCl, 3 M) in phosphate buffer (pH 7). A typical calibration plot from 25 g/dL to 1.5 mg/dL (R2 = 0.997) with a detection limit of 6.8 g/dL (S/N = 3) is achieved. The relative standard deviation of 21 successive injections of 0.2 mg/dL creatinine is 0.018. Under the optimal conditions, the frequently encountered biological interferences at physiological or higher concentration were investigated. Only uric acid revealed an obvious interference (298.1%). However, a Nafion® coated copper plating electrode shows a successful decrement of the interference of the uric acid with slightly decreased sensitivity of creatinine. The feasibility of this scheme for further clinical application is demonstrated by both HPLC and FIA to evaluate the creatinine concentration in a urine sample.
關鍵字 Creatinine; Enzyme-free; Structural specific amperometric scheme
語言 en
ISSN 0956-5663
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 Lin, Meng-Shan
審稿制度 是
國別 NLD
公開徵稿
出版型式 ,紙本
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