Purification and characterization of chitinase from a new species strain Pseudomonas sp. TKU008
學年 98
學期 2
出版（發表）日期 2010-06-01
作品名稱 Purification and characterization of chitinase from a new species strain Pseudomonas sp. TKU008
作品名稱（其他語言）
著者 Wang, San-Lang; Lin, Bo-Shyun; Liang, Tzu-Wen; Wang, Chuan-Lu; Wu, Pei-Chen; Liu, Je-Ruei
單位 淡江大學化學學系
出版者 Seoul: The Korea Science and Technology Center
著錄名稱、卷期、頁數 Journal of Microbiology and Biotechnology 20(6), pp.1001-1005
摘要 The chitinase-producing strain TKU008 was isolated from soil in Taiwan, and it was identified as a new species of Pseudomonas. The culture condition suitable for production of chitinase was found to be shaking at 30℃ for 4 days in 100 ml of medium containing 1% shrimp and crab shell powder, 0.1% K₂HPO₄, and 0.05% MgSO₄ㆍ7H₂O (pH 7). The TKU008 chitinase was suppressed by the simultaneously existing protease, which also showed the maximum activity at the fourth day of incubation. The molecular mass of the chitinase was estimated to be 40 kDa by SDS-PAGE. The optimum pH, optimum temperature, pH stability, and thermal stability of the chitinase were pH 7, 50℃, pH 6-7, and less than 50℃, respectively. The chitinase was completely inhibited by Mn²+ and Cu²+. The results of peptide mass mapping showed that 11 tryptic peptides of the chitinase were identical to the chitinase CW from Bacillus cereus (GenBank Accession No. gi 45827175) with a 32% sequence coverage.
關鍵字 Pseudomonas, chitinase, chitin, shrimp and crab shell wastes
語言 en
ISSN 1017-7825; 1738-8872
期刊性質 國外
收錄於 SCI
產學合作
通訊作者
審稿制度 是
國別 KOR
公開徵稿
出版型式 紙本
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