Utilization of squid pen for the efficient production of chitosanase and antioxidants through prolonged autoclave treatment
學年 97
學期 2
出版（發表）日期 2009-05-26
作品名稱 Utilization of squid pen for the efficient production of chitosanase and antioxidants through prolonged autoclave treatment
作品名稱（其他語言）
著者 Wang, San-lang; Wu, Pei-Chen; Liang, Tzu-wen
單位 淡江大學生命科學研究所
出版者 Kidlington: Pergamon
著錄名稱、卷期、頁數 Carbohydrate Research, 344(8), pp.979-984
摘要 We have developed a culture system for efficient production of chitosanase by Bacillus sp. TKU004. TKU004 was cultivated by using squid pen powder as the sole carbon/nitrogen source. The effects of autoclave treatments of the medium on the production of chitosanase were investigated. Autoclave treatment of squid pen powder for 45 min remarkably promoted enzyme productivity. When the culture medium containing an initial squid pen powder concentration of 3% was autoclaved for 45 min, the chitosanase activity was optimal and reached 0.14–0.16 U/mL. In addition, extracellular surfactant-stable chitosanase was purified from the TKU004 culture supernatant. The antioxidant activity of TKU004 culture supernatant was determined through the scavenging ability of DPPH, with 70% per mL. With this method, we have shown that marine wastes can be utilized efficiently through prolonged autoclave treatments to generate a high value-added product, and have revealed its hidden potential in the production of functional foods.
關鍵字 Chitosanase;Antioxidant;Squid pen wastes;Bacillus sp.;Autoclave treatment
語言 en
ISSN 0008-6215
期刊性質 國外
收錄於 SCI EI
產學合作
通訊作者 Wang, San-lang
審稿制度
國別 GBR
公開徵稿
出版型式 紙本
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