期刊論文
| 學年 | 110 |
|---|---|
| 學期 | 1 |
| 出版（發表）日期 | 2021-12-16 |
| 作品名稱 | Development of a novel liquid crystal Apta-sensing platform using P-shape molecular switch |
| 作品名稱（其他語言） | |
| 著者 | Asma Verdian; Zahra Khoshbin; Chih-Hsin Chen |
| 單位 | |
| 出版者 | |
| 著錄名稱、卷期、頁數 | Biosens. Bioelecton 2022 199, 113882 |
| 摘要 | Described herein is a liquid crystal (LC)-based aptasensor via employing the reorientation of LC triggered by the conformational changes of a P-shaped DNA structure. The structure consists of a short linker sequence as an immobilizer probe with ability to hybridize with the central part of the intact aptamer (Apt) sequence and an Apt terminal-locker (ATL) strand with complementary segments of the Apt terminal fragments. Bindings of two arm segments of the Apt sequence with the ATL strand enforces it to form a P-shaped configuration on the sensing platform. The selective interaction between the Apt strand and OTA leads to the disassembly of the Apt-ATL hybrid, collapse of the P-shaped structure, and consequently, transition of the optical appearance of the aptasensor texture. Determination of Ochratoxin A (OTA) in foods is an urgent demand in attempt to minimize food safety risks. To demonstrate the feasibility of our aptasensing design, the OTA specific aptamer was selected as a model. The developed LC aptasensor possesses a wide linear range from 0.01 aM to 100 pM, ultra-low limit of detection (LOD) of 0.0078 aM, and quantitative recoveries of 91–103.51% for OTA in rice and grape juice samples. This study proposes a novel and universal LC-based platform for facile, ultra-sensitive, and precision sensing of hazardous analytes in real samples. |
| 關鍵字 | Aptasensor;Grape juice analysis;Liquid crystal;Ochratoxin A;Rice analysis |
| 語言 | en |
| ISSN | 0956-5663 |
| 期刊性質 | 國外 |
| 收錄於 | SCI |
| 產學合作 | |
| 通訊作者 | |
| 審稿制度 | 是 |
| 國別 | TWN |
| 公開徵稿 | |
| 出版型式 | ,電子版,紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/123417 ) |