期刊論文
| 學年 | 104 |
|---|---|
| 學期 | 2 |
| 出版（發表）日期 | 2016-04-15 |
| 作品名稱 | Detecting trypsin at liquid crystal/aqueous interface by using surface-immobilized bovine serum albumin |
| 作品名稱（其他語言） | |
| 著者 | Cheng-Hao Chuang; Yi-Cheng Lin; You-Shuan Chen; Yu-Xun Chen; Chieh-Ming Chen; Hung Wei Shiu; Lo-Yueh Chang; Chia-Hao Chen; Chih-Hsin Chen |
| 單位 | |
| 出版者 | |
| 著錄名稱、卷期、頁數 | Biosensors and Bioelectronics 78, p.213–220 |
| 摘要 | We report a new mechanism for liquid crystal (LC)-based sensor system for trypsin detection. In this system, bovine serum albumin (BSA) was immobilized on gold grids as the enzymatic substrate. When the BSA-modified grid was filled with LC and immersed in the solution containing trypsin, the peptide bonds of BSA were hydrolyzed and peptide fragments were desorbed from the surface of gold grid, which disrupted the orientation of LC at the vicinity and resulted in a dark-to-bright transition of optical image of LCs. By using this mechanism, the limit of detection (LOD) of trypsin is 10 ng/mL, and it does not respond to thrombin and pepsin. Besides, the cleavage behavior on gold surfaces was directly visualized by the scanning photoelectron microscopy (SPEM), in particular for the chemical composition identification and element-resolved image. The loss of BSA fragments and the enhancement of Au photoelectron signal after trypsin cleavage were corresponding to the proposed mechanism of the LC-based sensor system. Because the signals reported by LC can be simply interpreted through the human naked-eye, it provides a simple method for fast-screening trypsin activity in aqueous solution. |
| 關鍵字 | Liquid crystal-based biosensor;Bovine serum albumin;Trypsin;Scanning photoelectron microscopy |
| 語言 | en |
| ISSN | 0956-5663 |
| 期刊性質 | 國外 |
| 收錄於 | SCI |
| 產學合作 | |
| 通訊作者 | Chih-Hsin Chen |
| 審稿制度 | 是 |
| 國別 | NLD |
| 公開徵稿 | |
| 出版型式 | ,電子版,紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/106198 ) |