期刊論文
| 學年 | 102 |
|---|---|
| 學期 | 2 |
| 出版（發表）日期 | 2014-03-27 |
| 作品名稱 | Liquid crystal-based immunoassay for detecting human serum albumin |
| 作品名稱（其他語言） | |
| 著者 | Chen, Chih-hsin; Ho, Wen-hsun |
| 單位 | 淡江大學化學學系 |
| 出版者 | Dordrecht: Springer Netherlands |
| 著錄名稱、卷期、頁數 | Research on Chemical Intermediates 40(6), pp.2229-2236 |
| 摘要 | Human serum albumin (HSA) is the most abundant protein in human blood plasma. It is commonly used as a biomarker in urine samples to identify the chronic kidney disease caused by high blood pressure or diabetes. In our research, a thin layer of liquid crystals (LCs) is used as a readout system for developing an immunoassay that reports the presence of HSA in the aqueous solution with optical signals. The detection principle of this assay is based on the variation of surface density of protein upon the specific binding of HSA on an anti-HSA immobilized surface, which leads a dark-to-bright transition of LC images under cross-polarizers. Our results show that the LC-based immunoassay can detect HSA at a concentration of 50 μg/mL. By using the slide with immobilized anti-HSA in array format, the concentration level of HSA can be simply determined by the number of LC spot shown on the slide. |
| 關鍵字 | Liquid crystal;Immunoassay;Human serum albumin;Microalbuminuria |
| 語言 | en |
| ISSN | 1568-5675 |
| 期刊性質 | 國外 |
| 收錄於 | SCI |
| 產學合作 | |
| 通訊作者 | Chen, Chih-Hsin |
| 審稿制度 | 是 |
| 國別 | NLD |
| 公開徵稿 | |
| 出版型式 | ,電子版,紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/100078 ) |