期刊論文
| 學年 | 102 |
|---|---|
| 學期 | 1 |
| 出版（發表）日期 | 2013-10-26 |
| 作品名稱 | Purification of chitinase/chitosanase from Bacillus cereus and discovery of an enzyme inhibitor |
| 作品名稱（其他語言） | |
| 著者 | Liang, Tzu-Wen; Chen, Yue-Yin; Pan, Po-Shen; Wang, San-Lang |
| 單位 | 淡江大學化學學系 |
| 出版者 | Amsterdam: Elsevier BV |
| 著錄名稱、卷期、頁數 | International Journal of Biological Macromolecules 63, pp.8-14 |
| 摘要 | A chitinase and a chitosanase were induced from a squid pen powder (SPP)-containing medium of Bacillus cereus TKU030 and purified by precipitation with ammonium sulphate and combined column chromatography. The purified chitinase and chitosanase exhibited optimum activity at 60 °C, pH 5–6 and 40 °C, pH 4, respectively. The chitinase and chitosanase were stable at 25–60 °C, pH 4–7 and 25–50 °C, pH 3–7, respectively. The chitinase and chitosanase showed the highest activity toward β-chitin and 60% DD chitosan, respectively. The chitinase was significantly inhibited by Mn2+ and EDTA but activated by Cu2+, Fe2+ and Ca2+. The chitosanase was significantly inhibited by Cu2+, Fe2+, Zn2+, Mn2+ and EDTA. The chitinase showed high stability in the presence of various surfactants, such as SDS, Tween 20, Tween 40 and Triton X-100. In contrast, these surfactants were inhibitors of the chitosanase. The chitinase and chitosanase were also inhibited by TKUPSP017, a small synthetic boron-containing molecule with a BF3K side-chain. However, TKUPSP017 enhanced the growth of B. cereus TKU030 in SPP-containing medium. |
| 關鍵字 | Bacillus cereus;Boron;Chitinase;Chitinase inhibitor;Chitosanase;Squid pen |
| 語言 | en |
| ISSN | 1879-0003 |
| 期刊性質 | 國外 |
| 收錄於 | SCI |
| 產學合作 | |
| 通訊作者 | Wang, San-Lang |
| 審稿制度 | 是 |
| 國別 | NLD |
| 公開徵稿 | |
| 出版型式 | ,電子版,紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/93053 ) |