期刊論文
| 學年 | 99 |
|---|---|
| 學期 | 2 |
| 出版（發表）日期 | 2011-03-01 |
| 作品名稱 | Identification of Paenibacillus sp. 2S-6 and application of its xylanase on biobleaching |
| 作品名稱（其他語言） | |
| 著者 | Ko, Chun-han; Tsai, Chung-hung; Tu, Jenn; Yang, Bing-yuan; Hsieh, Dong-lin; Jane, Wann-neng; Shih, Tzenge-lien |
| 單位 | 淡江大學化學學系 |
| 出版者 | London: Elsevier Ltd |
| 著錄名稱、卷期、頁數 | International Biodeterioration and Biodegradation 65(2), pp.334-339 |
| 摘要 | A Paenibacillus sp. strain 2S-6 was isolated from the black liquor of the first brownstock washing stage of kraft pulping process and identified by its 16S rDNA sequence. This bacterial strain utilized a variety of saccharides and polysaccharides as carbon source, but neither lignin nor lipids. Crude xylanase from Paenibacillus sp. 2S-6 was produced in a 5 L laboratory fermenter at 37 °C, pH 7. After 24 h, up to 10.5 IU xylanase per mg of protein in the crude extract of fermentation broth was obtained. After two-stage ultrafiltration, the optimal activity of partially purified xylanase reached 60.51 IU/mg at 50 °C, pH 6. A major band indicating molecular weight of 33 kDa was shown on SDS-PAGE for the partially purified xylanase. After 4 h at 60 °C, 48.99% and 31.25% residual xylanase activities were demonstrated at pH 7 and 9, respectively. Efficacy of its xylanase on the bleaching agent saving was demonstrated by using 5 IU xylanase per gram oven-dried pulp prior to bleaching, referred as biobleaching. Identical levels of brightness and higher levels of viscosity were obtained for the xylanase pretreated eucalypt kraft pulps followed by a 20% reduction of the bleaching agent dosage in the first step of a commercial C70/D30-Eo-D bleaching sequence. |
| 關鍵字 | Biobleaching; Bleaching pretreatment; Eucalypt; Kraft pulp; Paenibacillus; Xylanase |
| 語言 | en |
| ISSN | 0964-8305 |
| 期刊性質 | 國外 |
| 收錄於 | SCI EI |
| 產學合作 | |
| 通訊作者 | Ko, Chun-han |
| 審稿制度 | |
| 國別 | GBR |
| 公開徵稿 | |
| 出版型式 | 紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/61621 ) |