期刊論文
| 學年 | 97 |
|---|---|
| 學期 | 1 |
| 出版（發表）日期 | 2008-08-01 |
| 作品名稱 | Subunit fusion of two yeast D-amino acid oxidases enhances their thermostability and resistance to H2O2 |
| 作品名稱（其他語言） | |
| 著者 | Wang, Shih-juei; Yu, Chi-yang; Lee, Cheng-kang; Chern, Ming-kai; Kuan, I-ching |
| 單位 | 淡江大學生命科學研究所 |
| 出版者 | Dordrecht: Springer Netherlands |
| 著錄名稱、卷期、頁數 | Biotechnology Letters 30(8), pp.1415-1422 |
| 摘要 | D-Amino acid oxidases from Rhodosporidium toruloides and Trigonopsis variabilis (RtDAO and TvDAO) are both yeast homodimeric flavoenzymes. Two of their cDNA genes were connected by a hexanucleotide linker and heterologously expressed in E. coli to produce the corresponding double DAOs (dRtDAO and dTvDAO) with two subunits fused into a single polypeptide. The specific activities of double DAOs remained similar to those of native dimeric DAOs, although the catalytic efficiencies (kcat/KM) were decreased due to higher KM values. The Tm value for dRtDAO was shifted 5°C higher while that for dTvDAO was increased only by 2°C, in comparison with the corresponding native counterparts. In the presence of 10 mM H2O2, dRtDAO and dTvDAO exhibited half-lives of about 60 and 40 min, respectively, which were 2- and 1.5-fold, respectively, longer than their native DAOs. These yeast DAOs can therefore be thermally and oxidatively stabilized by linking their subunits together. |
| 關鍵字 | D-Amino acid oxidase; Gene fusion; Rhodosporidium toruloides; Stability; Trigonopsis variabilis |
| 語言 | en |
| ISSN | 0141-5492; 1573-6776 |
| 期刊性質 | 國外 |
| 收錄於 | SCI |
| 產學合作 | |
| 通訊作者 | Kuan, I-ching |
| 審稿制度 | |
| 國別 | NLD |
| 公開徵稿 | |
| 出版型式 | 紙本 電子版 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/61895 ) |