期刊論文
| 學年 | 99 |
|---|---|
| 學期 | 2 |
| 出版（發表）日期 | 2011-02-15 |
| 作品名稱 | Development of a label-free impedance biosensor for detection of antibody–antigen interactions based on a novel conductive linker |
| 作品名稱（其他語言） | |
| 著者 | Chen, Ching-sung; Chang, Ku-ning; Chen, Ying-hua; Lee, Chih-kung; Lee, Bryan Yong-jay; Lee, Adam Shih-yuan |
| 單位 | 淡江大學化學學系 |
| 出版者 | Amsterdam: Elsevier BV |
| 著錄名稱、卷期、頁數 | Biosensors and Bioelectronics 26(6), pp.3072-3076 |
| 摘要 | We developed a label-free impedance biosensor based on an innovative conductive linker for detecting antibody–antigen interactions. As the often used conventional long chain thiol is a poor conductor, it is not a suitable material for use in a faradaic biosensor. In this study, we adopted a thiophene-based conductive bio-linker to form a self-assembled monolayer and to immobilize the bio-molecules. We used cyclic voltammetry and impedance spectroscopy to verify the enhanced conductivity properties. Results showed that the electron transfer resistance of this new conductive linker was 3 orders of a magnitude lower than for a case using a conventional long chain thiol linker. With the decreased impedance (i.e. increased faradaic current), we can obtain a higher signal/noise ratio such that the detection limit is improved. Using fluorescence microscopy, we verified that our new conductive linker has a protein immobilization capability similar to a conventional long chain thiol linker. Also, using S100 proteins, we verified the protein interaction detection capability of our system. Our obtained results showed a linear dynamic range from 10 ng/ml to 10 μg/ml and a detection limit of 10 ng/ml. With our new conductive linker, an electrochemical impedance biosensor shows great potential to be used for point-of-care applications. |
| 關鍵字 | Impedance;Label-free;Conductive linker;S100; Point-of-care |
| 語言 | en |
| ISSN | 0956-5663 |
| 期刊性質 | 國外 |
| 收錄於 | SCI |
| 產學合作 | |
| 通訊作者 | Lee, Chih-kung |
| 審稿制度 | |
| 國別 | NLD |
| 公開徵稿 | |
| 出版型式 | 紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/58246 ) |