研究報告
| 學年 | 96 |
|---|---|
| 學期 | 1 |
| 出版（發表）日期 | 2008-01-01 |
| 作品名稱 | 新穎啤酒酵母菌蛋白質甲基轉移脢YJR129Cp之探討 |
| 作品名稱（其他語言） | Investigation of a Novel Protein Methyltransferase YJR129Cp from Saccharomyces cerevisiae |
| 著者 | 陳銘凱 |
| 單位 | 淡江大學生命科學研究所 |
| 描述 | 計畫編號：NSC97-2311-B032-001 研究期間：200808~200907 研究經費：720,000 |
| 委託單位 | 行政院國家科學委員會 |
| 摘要 | 本計劃是以本實驗室先前的發現為基礎。此發現已於C012-1中描述，證實了啤酒酵母菌之YJR129Cp為一真實具有活性之新穎蛋白質甲基轉移酶。此新發現利用一維電泳分析顯示，其蛋白質受質之分子量為95 kDa, 72 kDa, 55 kDa, 43 kDa，pI值為 6~7。本計劃將進一步利用蛋白質體學方法找出YJR129Cp之蛋白質受質；即進一步利用二維電泳分離及質譜分析來鑑定出蛋白質受質種類。並將利用定點突變、基因剔除、及甲基化反應等方式來證明是否YJR129Cp於體外及體內皆具有專一的活性。最後，計劃將延伸至功能方面的探討，此部分將借助於雙雜合篩選及合成致死基因篩選等技術。 This proposal is based on the previous finding in our lab that YJR129Cp of Saccharomyces cerevisiae is a bona fide, novel protein methyltransferase, as presented in C012-1. This new discovery indicates that the protein substrate has a molecular weight of 95 kDa, 72 kDa, 55 kDa, 43 kDa, and pI 6~7 in one-dimensional electrophoretic analysis. Here, identification of the protein substrate is planned by the proteomics approach; that is, two-dimensional electrophoretic separation followed by mass spectrometry. The specificity of both in vitro and in vivo activity of YJR129Cp is also planned to be verified using site-directed mutagenesis, gene deletion, and both in vitro and in vivo methylation assay. Finally, the study will be extended to the functional aspect by two-hybrid screening and synthetic lethal screening. |
| 關鍵字 | 甲基化; 蛋白質甲基轉移酶; 啤酒酵母菌; 蛋白質體學; methylation; protein methyltransferase; Saccharomyces cerevisiae; proteomics |
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| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/46931 ) |