期刊論文
| 學年 | 92 |
|---|---|
| 學期 | 1 |
| 出版（發表）日期 | 2003-10-08 |
| 作品名稱 | Reversible immobilization of lysozyme via coupling to reversibly soluble polymer |
| 作品名稱（其他語言） | |
| 著者 | Sz-Hau Chen; Yue-Horng Yen; Chuan-Lu Wang; San-Lang Wanga |
| 單位 | 淡江大學生命科學研究所 |
| 出版者 | Elsevier |
| 著錄名稱、卷期、頁數 | Enzyme and microbial technology 33(5), p.643-649 |
| 摘要 | Hen egg-white lysozyme (HEWL) can be covalently immobilized on a polymeric support (hydroxypropyl methylcellulose acetate succinate, AS-L), which is soluble above pH 5.5 but insoluble below pH 4.5. Commercial preparation of HEWL was used for the immobilization study. The immobilized enzyme (HEWL-AS) has much higher specific activity than those of free HEWL. The effects of pH and temperature on the activity and thermal stability of HEWL-AS were studied and compared with those of free HEWL. HEWL-AS retained nearly half of its initial chitinase activity even after heating at 70 °C for 50 min. The half-life at 4 °C was 4 days for free HEWL and 25 days for HEWL-AS. Following repeated pH cycles between 7 and 4.5, HEWL-AS retained nearly 50% of its original activity after 10 batches of chitinolytic reactions. |
| 關鍵字 | Lysozyme;Chitinase;Reversibly soluble polymers;Immobilized enzyme |
| 語言 | en |
| ISSN | 0141-0229 |
| 期刊性質 | 國外 |
| 收錄於 | |
| 產學合作 | |
| 通訊作者 | |
| 審稿制度 | 否 |
| 國別 | USA |
| 公開徵稿 | |
| 出版型式 | ,電子版 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/18857 ) |